[3f83a] %Read* Bovine Trophoblast Protein-1: Chemical Characteristics and Antiluteolytic Effects on Uterine and Ovarian Function - Stephen Helmer #P.D.F!
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In yet other aspects, the invention relates to essentially pure bovine trophoblast protein-1, to means and methods of providing this protein by recombinant dna technology and of converting recombinant btp-1 to novel entities, such as pharma ceutical compositions, useful for the prophylactic of therapeutic treatment of other species of warm.
Ovine trophoblastic protein-1 (otp-1), an early secretory protein of the sheep blastocyst, was purified after culturing day 14–16 conceptuses for 24 h in vitro. The localization of otp-1 in the pregnant day 16 sheep uterus was determined immunocytochemically.
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Bovine trophoblast protein-1 (btp-1) is a 172-amino acid interferon-α that has a role in maternal recognition of pregnancy in cattle. Here we describe production of btp-1 by recombinant procedures in escherichia coli.
Keywords: bovine; blastocyst; elongation; pre-implantation; trophoblast; epiblast; differentiation mrna (nucleosome-associated-protein 1) was preferentially.
Expression of antiviral activity and induction of 2',5'-oligoadenylate synthetase by conceptus secretory proteins enriched in bovine trophoblast protein-1 march 1991 biology of reproduction 44(2.
Inhibition of lymphocyte proliferation by ovine trophoblast protein-1 and a high molecular weight glycoprotein produced by the peri-implantation sheep conceptus am j reprod immunol 1989 mar;19(3):99-107.
Abstract: bovine conceptuses exert an antiluteolytic (anti-pgf-2 alpha) effect on the uterus by decreasing the secretion of pgf that results in maintenance.
A novel role for gm-csf: enhancement of pregnancy specific interferon production, ovine trophoblast protein-1 localization of insulin-like growth factor-i (igf-i) and -ii messenger ribonucleic acid and type 1 igf receptors in the ovine uterus during the estrous cycle and early pregnancy.
Effects of uric acid on trophoblast integration into endothelial cell monolayers. We examined the effects of uric acid on the degree of trophoblast cells integration into uterine microvascular endothelial cell monolayers. 5, 3, 5, or 7 mg/dl) for 2 h and then coincubated with trophoblast.
Progesterone is also involved in the stimulation of the uterine secretions that regulate conceptus growth and the release of the bovine trophoblast protein-1 (btp-1) necessary for inhibiting endometrial pgf2 alpha release.
Bovine trophoblast protein-1 (btp-1), which cross-reacts immunologically with otp-1, was also detectable specifically in the uterine flushings of pregnant cows when anti-otp-1 antiserum was used.
The antiluteolytic signal in bovine conceptus secretory proteins (bcsp) is bovine trophoblast protein-1 (btp-1), a group of 7 isomers of n-linked glycoproteins in two size classes (22,000 high-mannose and 24,000 complex types) that are related immunologically to ovine trophoblast protein-1 (otp-1).
Ovine trophoblast protein 1, an early secreted blastocyst protein, binds specifically to uterine endometrium and affects protein synthesis. Characterization of endometrial receptors for ovine trophoblast protein-1 during the estrous cycle and early pregnancy in the sheep.
Ə l j ʊəˈr ɒ n ɪ k /; abbreviated ha; conjugate base hyaluronate), also called hyaluronan, is an anionic, nonsulfated glycosaminoglycan distributed widely throughout connective, epithelial, and neural tissues.
As a member of the wwpdb, the rcsb pdb curates and annotates pdb data according to agreed upon standards. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Ovine trophoblast protein 1 (otp-1) is the interferon alpha (ifn-alpha) variant with potent antiviral activity and low toxicity that is responsible for maternal.
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Bovine trophoblast protein-1 (btp-1) is a 172-amino acid interferon- alpha that has a role in maternal recognition of pregnancy in cattle.
Paracrine hormone primarily responsible for maternal recognition of pregnancy. Interacts with endometrial receptors, probably type i interferon receptors, and blocks estrogen receptor expression, preventing the estrogen-induced increase in oxytocin receptor expression in the endometrium.
Molecular cloning and characterization of complementary deoxyribonucleic for vitellogenin and growth hormone are regulated by es- acids corresponding to bovine trophoblast protein-i: a comparison with ovine trogen [26, 27] and glucocorticoids [28], respectively.
The protein concentration of each lot of bovine pituitary extract is determined by the bradford method.
The trophoblast plugs block spiral arteries until the beginning of the second trimester (11-14 weeks). Msx2 induces trophoblast invasion in human placenta normal implantation depends on appropriate trophoblast growth and invasion. Meanwhile, overexpression of msx2 and treatment with the msx2-specific sirna resulted in decreased and increased.
The present study was designed to test the effect of bovine conceptus secretory proteins (bcsp) in general and the bovine trophoblast protein-1 complex (btp-1) in particular on endometrial function.
Ovine and bovine trophoblast protein-1 (otp-1 and btp-1) have been strongly implicated as antiluteolytic agents and responsible for maternal recognition of pregnancy in sheep and cattle, respectively.
The production, purification, and bioactivity of recombinant bovine trophoblast protein-1 (bovine trophoblast interferon.
In other species such as cattle and sheep, trophoblast has furthermore been shown to produce unique ifns [bovine trophoblast protein-1 (btp-1) and ovine trophoblast protein-1 (otp-1)] in the early stage of pregnancy which apart from their antiviral and antiproliferative properties, similar to the already known ifns, also initiate maternal.
Mar 4, 2021 chemical characteristics of p46oscc protein and mrna identified in rat the availability of a rat trophoblast cell line (rcho-1) has provided a useful in bovine serum (fbs) as previously reported (faria and soares,.
This invention provides bovine pregnancy test methods and devices. The test is also suitable for other ruminant and/or ungulate animals. Antigens from group a (early pregnancy antigens), and/or group b (mid-pregnancy antigens), and group c (early, mid- and late pregnancy antigens) are detected in a fluid from the animal, and pregnancy is reliably determined.
Bovine and ovine pregnancy-associated glycoproteins-1 are products of binucleate trophoblast cells and belong to the aspartic proteinase gene family bovine pag 2, unlike bovine pag 1, has a catalytic center with a consensus sequence of amino acids.
Nov 5, 2020 pdf background secreted protein of ly-6 domain 1 (sold1), a secretory-type member of the ly-6 sold1 is expressed in bovine trophoblast cell lines and regulates cell invasiveness or immunocytochemistry.
Dickkopf-related protein 1 is a progestomedin acting on the bovine embryo during the morula-to-blastocyst transition to program trophoblast elongation.
In order to help elucidate the process of epiblast and trophoblast cell differentiation in bovine embryos invitro, we attempted to develop a suitable culture medium to allow extended embryo culture. Day 7 bovine blastocysts developed in conventional medium were cultured further in embryonic stem cell medium with or without leukaemia inhibitory.
Elsevier scientific key words: bovine trophoblast protein-l (btp-1) complex; glycoprotein.
Background bovine trophoblast binucleate cells (bnc) express a plethora of molecules including bovine placental lactogen (bpl, gene name is bcsh1) and bovine prolactin-related protein-1 (bprp1). Bcsh1 and bprp1 are members of the growth hormone (gh)/prolactin (prl) gene family, which are expressed simultaneously in bnc and are central to placentation and the progression of pregnancy in cattle.
Bovine trophoblast protein-1 (btp-1) is a secreted glycoprotein that consists of several forms differing slightly in mol wt and isoelectric point. It is produced by bovine conceptuses after about day 15 of pregnancy and is believed to play a key role in signalling the presence of an embryo to the mother.
Jan 17, 2020 in the bovine placenta, intimate fetomaternal contacts are restricted to discrete gene ontology (go) analyses revealed that molecular functions and protein-1 genes in the bovine placenta and uterus during pregnanc.
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